Journal: Journal of molecular biology

Article Title: Impact of xenogeneic silencing on phage-host interactions

doi: 10.1016/j.jmb.2019.02.011

Figure Lengend Snippet: Silencing of phage elements in bacterial genomes.

Article Snippet: An overview of the studies showing the influence of XS proteins on the control of phage gene expression is provided in . table ft1 table-wrap mode="anchored" t5 caption a7 Type of silencer Host strain GC of host (%) Prophage-like element Length (kb) of phage GC (%) of phage Reference H-NS E. coli K-12 BW25113 50.8 Rac (cryptic) 23.1 47.1 [ 56 ] S. onediensis MR-1 45.9 CP4So (cryptic) 36 43 [ 59 ] MvaT P. aeruginosa PAO1 66.6 Filamentous phage Pf4 15.7 58.7 [ 54 , 55 ] Rok B. subtilis 168 43.5 Prophage region 4 8 35.8 [ 24 ] Prophage region 5 20.7 37.5 Prophage region 6 34.8 36.1 SPβ 134.4 34.6 Lsr2 C. glutamicum ATCC 13032 53.8 Cryptic prophage CGP1 13.5 47.1 [ 9 ] Cryptic prophage CGP3 186.0 48.4 M. tuberculosis H37Rv 65.6 Prophage region 1, Rv1573-1588c, (Rv1582c) 10.5 (1.4 * ) 66.2 (62.5 * ) [ 38 ] Prophage region 2, Rv2645-2664, (Rv2658-2659c) 12.3 (1.5 * ) 66.2 (63.5 * ) Open in a separate window * In case of M. tuberculosis also the bound genes were considered and are indicated in brackets. caption a8 Silencing of phage elements in bacterial genomes.

Techniques:

Journal: BMC Microbiology

Article Title: Quinone-dependent D-lactate dehydrogenase Dld (Cg1027) is essential for growth of Corynebacterium glutamicum on D-lactate

doi: 10.1186/1471-2180-10-321

Figure Lengend Snippet: List of bacterial strains, plasmids and oligonucleotides

Article Snippet: Based on the 5'- and 3'- sequences of dld (accession no. YP_225194) in the genomic DNA of Corynebacterium glutamicum ATCC 13032, the oligonucleotides dld1 and dld2 were designed, and dld was amplified by PCR from the genomic DNA of C. glutamicum ATCC 13032 (1 ng) with dld1 and dld2 (0.2 pmol).

Techniques: Plasmid Preparation, Sequencing, Mutagenesis, Binding Assay